AQ97 High Fidelity DNA Polymerase 2U/µl, 5x AQ97 Buffer and

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25 mM MgCl2, 100 U (1 tube x 0.05 ml)
High fidelity proof-reading DNA Polymerase featuring robust amplification on AT-rich, GC-rich and long DNA targets. Recommended for cloning and mutagenesis.

AQ97 High Fidelity DNA Polymerase 2 U/µl is a proofreading DNA polymerase displaying the following features; high fidelity >60x Taq DNA Polymerase, fast amplification due to its high elongation rate, ability to amplify problematic DNA targets, such as those with low to high GC content and ability to perform amplification of long DNA targets. These features enables accurate and reliable PCR results.

AQ97 High Fidelity DNA Polymerase is recommended for applications, which require extremely high fidelity such as cloning/Sub-cloning, NGS applications, SNP analysis and mutagenesis.

FEATURES

• High fidelity: >60x Taq Fidelity
• High elongation rate: 10 sec/kb
• 3’→5’ proofreading exonuclease activity
• Long range capacity: 18 kb for gDNA and 25 kb for λ DNA
• Good coverage on DNA templates with low to high GC content

DESCRIPTION

AQ97 High Fidelity DNA Polymerase exhibits both 5'→3' DNA polymerase activity and 3'→5' proofreading exonuclease activity enabling this polymerase to correct base pair mismatches. The DNA binding domain of this polymerase, ensures the excellent high fidelity, long range capacity and also fast amplification results.

The AQ97 High Fidelity DNA Polymerase kit is supported with 5x AQ97 Buffer allowing robust amplification on DNA targets with low to high GC content and long DNA targets. The kit also includes an extra tube of MgCl₂ in 25 mM concentration if adjustment of PCR conditions is required.

For difficult amplicons, such as GC-rich DNA samples, those with complex secondary structures or longer amplicons, the addition of 1 – 2 M Betaine Enhancer Solution is recommended.

For more convenient handling AQ97 High Fidelity DNA Polymerase is also available as a 2x master mix.

FIDELITY

The fidelity of a polymerase refers to its ability to insert the correct base during PCR. On the contrary, the rate of misincorporation is known as a polymerase error rate.  Fidelity depends on the polymerase, the buffer system in use and the quality of your template DNA.

For more information about fidelity read here.