Ampliqon Taq DNA Polymrase 50 U/µl glycerol free, without Buffer, 250000 U

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(1 tube x 5 ml)
For automation and freeze drying

FEATURES

• Glycerol free storage buffer
• Highly concentrated formats (50 U/µl & 100 U/µl)
• Ideal for PCR kit manufactures
• Processes up to 5 kb
• dUTP incorporation possible
• Leaves a 3'dA overhang

 

APPLICATIONS

• Lyophilization
• Automated high throughput testing
• Suitable for robot-aided pipetting
• DNA target detection

DESCRIPTION

Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'→3'dA DNA polymerase and a 5'→3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.

Highly concentrated Taq DNA Polymerase Glycerol Free is ideal for PCR kit manufactures and DNA testing facilities, requiring large amounts of DNA polymerases. The highly concentrated Taq DNA Polymerase Glycerol Free 50 U/µl & 100 U/µl allows for concentrated bulk production of PCR master mixes. Concentrated bulks in glycerol free formats are attractive when using automated pipetting robots and other applications where accurate pipetting of small volumes is crucial. Furthermore, concentrated master mixes are easier to lyophilize due to the reduced volume of the concentrated mix. Lyophilized amplification mixtures are often included in commercial PCR kits for diagnostic purposes, such as detection of pathogens, diseases and gene expression assays.