Ampliqon Taq DNA Polymerase 5 U/µl glycerol free,
10x Ammonium Buffer and MgCl2, 500 Unit 1 tube x 0.1 ml
Ampliqon Taq DNA Polymerase is developed for automation and freeze drying. It is a glycerol free formulation of standard Ampliqon Taq DNA Polymerase and is well suited for automated routine PCR applications, or where accurate pipetting of small amounts is crucial.
FEATURES Glycerol free storage buffer Suitable for automated high throughput testing Suitable for applications involving freeze drying Suitable for robot-aided pipetting High product yield Processes up to 5 kb dUTP incorporation possible Leaves a 3'dA overhang
DESCRIPTION Ampliqon Taq DNA Polymerase Glycerol Free exhibits both a 5'-3'dA DNA polymerase and a 5'-3' exonuclease activity. The 5'-3' exonuclease activity leaves a 3' overhang on the PCR product, which are convenient for direct T-A cloning. Taq DNA Polymerase Glycerol Free lacks 3'-5' exonuclease activity and proofreading ability.
WHAT GLYCEROL DOES Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.