PureIT ExoZAP PCR Clean-Up, 1x1.0ml, 500 Reaktionen

PureIT ExoZAP PCR Clean-Up, 1x1.0ml, 500 Reaktionen
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Bestell-Nr.: A620603 PureIT ExoZAP PCR Clean-Up, 1x1.0ml, 500 Reaktionen 360,00 € 288,00 € für 1 Verpackung zzgl. gesetzl. MwSt. (20%) zzgl. Versandkosten

One-step PCR Clean-up for optimal sequencing results. Clean-up results in just 15 minutes.


  • One-step PCR product clean up
  • Fast 15 min protocol
  • No need for spin columns or magnetic beads
  • Treatment improves downstream applications such as DNA sequencing and SNP analysis
  • Minimal loss of PCR products during treatment

PureIT ExoZAP PCR CleanUp consists of a balanced combination of a heat labile Exonuclease (HL-ExoI) I and a recombinant Shrimp Alkaline Phosphatase (rSAP).

Treatment of amplified PCR products with PureIT ExoZAP PCR CleanUp, removes residual primers, single-stranded DNA and inactivates excess dNTPs by dephosphorylation. Add PureIT ExoZAP PCR CleanUp directly to the reaction containing the amplified PCR product. After treatment at 37 °C for 5 minutes PureIT ExoZAP PCR CleanUp is completely inactivated by heating at 80 °C for 10 minutes.


This protocol serves as a guideline for clean-up of 5 μl PCR product using PureIT ExoZAP PCR CleanUp*.

  • Take out PureIT ExoZAP PCR CleanUp from the -20 °C freezer.
  • Keep PureIT ExoZAP PCR CleanUp on ice at all times.
  • Add 2 μl PureIT ExoZAP PCR CleanUp** to 5 μl of amplified PCR product.
  • Incubate the reaction at 37 °C for 5 minutes to degrade remaining primers, single-stranded DNA and to inactivate excess nucleotides by dephosphorylation.
  • Incubate at 80 °C to completely inactivate PureIT ExoZAP PCR CleanUp.
  • The cleaned up PCR product can now be used for downstream applications such DNA sequencing, primer extension experiment or SNP analysis.
  • After treatment the PCR products can be stored at -20 °C

*If treating PCR product of higher volume, then increase proportionally the amount of PureIT ExoZAP PCR CleanUp.

**PureIT ExoZAP PCR CleanUp works in PCR buffers.