TEMPase Hot Start DNA Polymerase 5 U/µl glycerol free, 10x Ammonium Buffer...

TEMPase Hot Start DNA Polymerase 5 U/µl glycerol free, 10x Ammonium Buffer...

Die Abbildung kann abweichen und Zubehör enthalten, das nicht im Lieferumfang dieses Artikels enthalten ist.

 
Bestell-Nr.: A241103 TEMPase Hot Start DNA Polymerase 5 U/µl glycerol free, 10x Ammonium Buffer... 77,00 € für 1 Verpackung zzgl. gesetzl. MwSt. (20%) zzgl. Versandkosten
TEMPase Hot Start DNA Polymerase 5 U/µl glycerol free, 10x Ammonium Buffer and MgCl2, 500 Unit 1 tube x 0.1 ml TEMPase Hot Start DNA Polymerase Glycerol Free is a glycerol free formulation of our regular TEMPase Hot Start DNA Polymerase and is well suited for automated high throughput testing, freeze drying or where accurate pipetting of small volumes are crucial FEATURES Convenient reaction set up at room temperature Automated high throughput testing Freeze drying dUTP incorporation possible Increased sensitivity, specificity and product yield DESCRIPTION TEMPase Hot Start DNA Polymerase Glycerol Free is a chemically modified version of Ampliqon Taq DNA Polymerase and is activated by heat treatment. A chemical moiety is attached to the enzyme at the active site, which renders the TEMPase Hot Start enzyme inactive at room temperature. During set up and the first ramp of thermal cycling, the enzyme is not active and misprimed primers are not extended. Once the PCR reaction reaches the optimal activation temperature, the chemical moiety is cleaved releasing the active TEMPase Hot Start DNA Polymerase, resulting in higher specificity, sensitivity and yield compared to standard Ampliqon Taq DNA polymerase. Glycerol is normally a major part of the storage buffer of enzymes and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, hence stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast, robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.
mehr