Taq DNA Polymerase 5 U/µl, 10x Cmbination Buffer and MgCl2, 5000 Unit
Taq DNA Polymerase 2x Master Mix RED 1.5 mM MgCl2 (final concentraion), 100 Reactions - for direkt loading
2 tubes x 1.25 ml
Taq DNA Polymerase Master Mix RED is a very popular alternative to Taq DNA Polymerase Master Mix with the same advantages. The inert red dye and stabiliser are extra features, which allow direct loading onto your agarose gel for analysis.
FEATURES Premixed solution Minimal optimisation Direct loading onto agarose and SDS DNA gels Easy visualisation of pipetting Dye front runs at 300 - 1000 bp on 0.5 - 1.5 % agarose gel No need for a separate loading dye Increased reproducibility High product yield dUTP incorporation possible Processes up to 5 kb Leaves a 3'dA overhang
DESCRIPTION Taq DNA Polymerase Master Mix RED is a ready to use 1.1x or 2x master mix. Taq DNA Polymerase Master Mix RED is available in two final MgCl2 concentrations: 1.5 mM and 2.0 mM. Taq DNA Polymerase Master Mix RED is composed of Ampliqon Taq DNA Polymerase, the NH4+ buffer system, dNTPs and magnesium chloride. The red dye and the stabiliser do not interfere with the PCR. If necessary, the red dye can be removed from the PCR product by spin column purification or other methods. Furthermore, PCR products generated using Taq DNA Polymerase Master Mix RED DNA, can directly be used for sequencing after treatment with either spin columns or ExoSAP-IT. For information about the ExoSAP-IT protocol please contact us.